Logo-jrip
Submitted: 08 Feb 2024
Accepted: 15 Apr 2024
ePublished: 21 Apr 2024
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)

J Renal Inj Prev. 2024;13(2): e34306.
doi: 10.34172/jrip.2024.34306
  Abstract View: 128
  PDF Download: 56

Original

A comparative study of true and pseudo-peroxidase and their relative biomarkers between male and female patients with chronic kidney disease

Yamama Zuhair Hani* ORCID logo, Israa Ghassan Zainal ORCID logo

1 Chemistry Department, College of Science, University of Kirkuk, Kirkuk, Iraq
*Corresponding Author: Yamama Zuhair Hani, Email: yamama1986@uokirkuk.edu.iq

Abstract

Introduction: Pseudo-peroxidase enzymes, despite mimicking the catalytic prowess of true peroxidases, lack authentic enzymatic functionality. The critical discernment between pseudo and true peroxidases is imperative when assessing oxidative biomarkers in both sexes, as variations in enzymatic activity may underpin gender-specific disparities in oxidative stress profiles, potentially impacting disease vulnerability and therapeutic modalities.

Objectives: This study aims to ascertain true and pseudo peroxidase activity alongside other oxidative stress biomarkers, comparing these parameters between male and female patients afflicted with chronic kidney disease (CKD).

Patients and Methods: A total of 140 blood samples were analyzed, comprising 80 from CKD patients and 60 from healthy controls. Various parameters, including total protein, albumin, glomerular filtration rate (GFR), urea, creatinine, hemoglobin (Hb), free amino acids, globulins, ischemia-modified albumin (IMA), carbonyls, total thiols, native thiols, disulfides, peroxidase, and pseudo peroxidase activity, as well as specific activity, were assessed in both groups.

Results: Females with CKD exhibited a significant elevation in numerous serum parameters, including total protein, albumin, globulins, IMA, GFR, urea, creatinine, free amino acids, carbonyls, and disulfides compared to their male counterparts with CKD. Conversely, males with CKD demonstrated a notable increase in peroxidase activity, peroxidase specific activity, pseudo peroxidase activity, and pseudo peroxidase specific activity in comparison to females with CKD.

Conclusion: The comparative analysis between male and female CKD patients regarding the assessed parameters revealed notable differences in albumin, total protein, GFR, urea, creatinine, Hb, free amino acids, globulins, IMA, carbonyls, total thiols, native thiols, disulfides, peroxidase, and pseudo peroxidase activity and specific activity.


Implication for health policy/practice/research/medical education:

Pseudo-peroxidase enzymes imitate true peroxidases; however, they are deficient in authentic enzymatic functionality, which is indispensable for accurately evaluating oxidative biomarkers in both male and female subjects. Females with chronic kidney disease showed higher oxidative stress serum parameters than males.

Please cite this paper as: Zuhair Hani Y, Ghassan Zainal I. A comparative study of true and pseudo-peroxidase and their relative biomarkers between male and female patients with chronic kidney disease. J Renal Inj Prev. 2024; 13(2): e34306. doi: 10.34172/jrip.2024.34306.

First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 129

Your browser does not support the canvas element.


PDF Download: 56

Your browser does not support the canvas element.